Optimization of Fermentation Medium for Endoinulinase Production by Response Surface Analysis

doi:10.7506/spkx1002-6630-201313040

Abstract

Abstract:

The production of endoinulinase by Aspergillus niger in submerged cultivation was studied. Burdock was used
as the only carbon source. Among 20 Aspergillus niger strains tested, strain 08013 exhibited the highest endoinulinase
activity of 3.27 U/mL in the fermentation supernatant. Preliminary results from this study showed that the optimal
fermentation time, metal ion, carbon source, organic nitrogen source and inorganic nitrogen source were 108 h, 0.5 g/100 mL of
sodium ion, burdock, 2 g/100 mL of yeast extract, 2 g/100 mL of (NH4)2HPO4, respectively. Response surface Box-
Behnken design was used to optimize burdock, yeast extract and (NH4)2HPO4 concentration. A mathematical model
was developed and analyzed indicating the effect of the studied variables on endoinulinase activity. The optimal culture
medium for producing endoinulinase contained 9.98% of burdock juice, 1.74 g/100 mL of yeast extract and 1.82 g/100 mL
of (NH4)2HPO4. The maximum predicted endoinulinase activity under the optimized conditions was 7.23 U/mL, which
was close to the experimental value of 7.26 U/mL. Thus the regression model developed in this study had good prediction
ability. This experimental value exhibited an increase of 122% compared with that obtained before optimization.

Key words: inulinase, Aspergillus niger, fermentation medium, optimization, response surface analysis

CLC Number:

TS 201.3

CAO Ze-hong，QIN Wei-dong，LI Chao，DONG Yu-wei，SHANG Xue-bing，WANG Wei-dong，YANG Wan-gen. Optimization of Fermentation Medium for Endoinulinase Production by Response Surface Analysis[J]. FOOD SCIENCE, 2013, 34(13): 186-192.

References

[1] 韦红群, 邓建珍, 曹建华, 等. 黑曲霉( Aspergillus niger )产菊粉酶发酵条件的研究[J]. 食品工业科技, 2008 (2) : 9-11.

[2] Viswanathan P, Kulkarni PR. Full factorial design to study fermemtative production of inulinases using inulin from kuth ( saussurealappa) root powder by Aspergillus niger van teighemu UV11 mutant [J]. Bioresource Technology, 1995, 54: 117-121.

[3] Gouda MK. Some properties of inulinase from Aspergillus fumigatus [J]. Biological Sciences, 2002, 5 (5) : 589-593.

[4] Uchiyama T. Action of A rthrobacter ureafaciens inulinase II on several oligofructans and bacterial levans [J]. Biochimica Biophysica Acta, 1975, 397: 153-163.

[5] 王琳, 刘兆普, 赵耕毛, 等. 产菊粉酶菌株的筛选及其产酶条件的优化[J]. 南京农业大学学报, 2007, 30 (2) : 73-77.

[6] Castro GR, Baigori MD, Sineriz F. A plate technique for screening of inulin degrading microorganisms [J]. Microbiological Methods, 1995, 22: 51 -56.

[7] Parekh S, Margaritas A. Appl Microbial Biotechnol, 1988, 22: 446-448.

[8] 唐艳斌, 许波, 唐湘华, 等. 产菊粉酶曲霉菌株的筛选和发酵条件的优化[J]. 饲料研究,2008 (7):32-35.

[9] 华承伟, 王建华, 滕达, 等. 菊粉化学和微生物菊粉内切酶研究进展[J]. 中国食品学报, 2004, 4(4): 103-108.

[10] Pambati Cayyanna. Optimizing medium constituents and fermentation condition for citric acid from palmura jaggeryusing response surface method [J]. World Journal of Micri biology & Biotechnology, 2001, 17: 331-335.

[11] Lailst, Pan CC, Tzeng BK. The influence of medium design on lovastatin production and pellet formation wethahigh producing mutant of Aspergillus terreus in submerged cultures [J]. Proc Biochem, 2003, 38: 1314-1326.

[12] A. Azaola, P. Bustarnante, S. Huertueta.l Use of response surface method o logy to describe biomass production of Bifidobacterium infantis in complex media [J]. Biotechnology Techniques, 1999, 13: 93- 95.

[13] Chen QH, He GQ, Mokhtar AMA. Optimization of medium composition for the production of elastase by Bacillussp. EL 31410 with response surface methodology [J]. Enzyme Microb Tech, 2002, 30: 667- 672.

[14] 李云雁, 胡传荣. 试验设计与数据处理(第二版)[M]. 北京:化学工业出版社, 2009,199-201.

[15] 陈晓明, 金征宇. 菊粉降解生产低聚果糖[J]. 饲料工业, 2000, 21(11) : 29-31.

[16] 北京大学生物系生物化学教研室. 生物化学实验指导[M]. 北京:高等教育出版社,1979, 122 -241.

[17] 李莹, 江波, 金征宇. 棉纤维固定化菊粉酶酶学性质及连续降解菊糖的研究[J]. 食品工业科技, 2006 (3) : 67-70.

[18] 袁玉荪, 朱婉华, 陈钧辉. 生物化学实验(第二版) [M]. 北京:高等教育出版社, 1988, 118-121.

[19] 黄森, 查学强, 罗建平, 等. Box-Behnken法优化提取霍山石斛活性多糖的研究[J]. 中药材, 2007, 30(5): 591-594.

[20] 李娟, 陆洋, 王广基. Box-Behnken效应面法优化甘草次酸长循环脂质体的处方[J]. 中国药科大学学报, 2007, 38(2):120-124.

[21] 沈娟, 陆兆新, 别小妹, 等. Bacillus sp. fmbJ224发酵产新型抗菌肽发酵条件的优化研究[J]. 生产与科研经验, 2010, 36, (2):123-127.