FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (20): 128-131.

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Optimization of Extraction Process for Polysaccharides from Stems and Leaves of Gypsophila oldhamiana Miq. by Response Surface Methodology

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  • Received:2012-06-06 Revised:2012-09-13 Online:2012-10-25 Published:2012-11-09

Abstract: Response surface methodology was employed to optimize conditions for the extraction of polysaccharides from the stems and leaves of wild Gypsophila oldhamiana  Miq. On the basis of single-factor experiments, temperature, time and  solvent-to-solid ratio were selected out of four extraction conditions for a three-level Box-Behnken experimental design. Results showed that optimal extraction conditions were obtained using two extraction cycles at 97 ℃ with distilled water at a solvent-to-solid ratio of 30.5:1 (mL/g) for 3.5 h. Under these conditions, the predicted and experimental extraction yields of polysaccharides were 2.226% and 2.219%, respectively.

Key words: Gypsophila oldhamiana Miq, polysaccharides, response surface methodology, extraction

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