FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (20): 138-143.

Previous Articles     Next Articles

Crude Polysaccharides from Suaeda salsa L.: Optimization of Extraction Process Using Response Surface Methodology and Antioxidant Activity

  

  • Received:2012-06-14 Revised:2012-09-05 Online:2012-10-25 Published:2012-11-09

Abstract: Response surface methodology was used to optimize the extraction of crude polysaccharides from Suaeda salsa L. Three extraction parameters including temperature, time and solvent-to-solid ratio were identified as main factors affecting the extraction yield of crude polysaccharides. The extraction parameters were optimized by a three-variable, three-level Box-Bohnken experimental design combined with response surface analysis. The optimal conditions for polysaccharide extraction from Suaeda salsa L. were 3.5 h extraction with hot water (87 ℃) at a solvent/solid ratio of 31.5 mL/g, resulting in an extraction yield of polysaccharides of 2.028%. As demonstrated by comparison with VC and TBHQ, crude polysaccharides from S. salsa L. had excellent reducing power and high abilities to scavenge superoxide anion and hydroxyl free radicals with IC50 values of 0.84 mg/mL and 0.71 mg/mL, respectively. The antioxidant effect of crude polysaccharides from S. salsa L. was lower than that of VC but similar to that of TBHQ.

Key words: Suaeda salsa L., polysaccharides, response surface methodology, antioxidant activity

CLC Number: