Optimized Conditions of Trichloroacetic Acid Precipitation for the Determination of Free Amino Acids in Fermented Soybean Paste

doi:10.7506/spkx1002-6630-201310004

Abstract

Abstract:

In this study, trichloroacetic acid precipitation for protein removal coupled with biuret colorimetry was applied
to determine protein in fermented soybean paste. The influence of three factors such as precipitant dosage and concentration,
temperature and centrifuge speed was examined, and aa mathematical model was established by Box-Behnken design
for statistical optimization of the factors by response surface analysis. The results showed that the optimal conditions for
trichloroacetic acid precipitation were TCA concentration 8.3 g/100 mL, precipitation temperature 47 ℃, and centrifugal
speed 1726 r/min and had the smallest effect on the determination of free amino acids. The precipitate obtained under these
conditions contained 23.29 g/100 g prtoein.

Key words: fermented soybean paste, amino acid, protein, precipitation, response surface analysis

CLC Number:

TS 207. 2

ZHANG Miao，WU Jun-rui，DAI Jin-yue，QIU Xiao-yu，YUE Xi-qing. Optimized Conditions of Trichloroacetic Acid Precipitation for the Determination of Free Amino Acids in Fermented Soybean Paste[J]. FOOD SCIENCE, 2013, 34(10): 16-20.

References

包启安. 酱及酱油的起源及其生产技术(一)[J]. 中国调味品, 1992(9):1-4.

廖杰. 论酱油风味、香气的产生及形成[J].中国调味品，2004（10）：5-7.CARERI M, MANGIA A, BARBIERI G, et a1. Sensory property relationship to chemical data of Italian-type dry -cured ham[J]. Journal of Food Science, 1993, 58(5): 968-972 .

钟秋瓒.乳饮料及乳制品中游离甘氨酸的测定[D].食品与发酵工业,2009,35(2):159-161.

GB/T5009.124-2003. 食品中氨基酸的测定[S] . 北京：中华人民共和国卫生部，中国国家标准化管理委员会，2003.

刘莹，傅泽田，侯彩云，等. 人为添加三聚氰胺液态乳中蛋白质含量测定方法的研究[J]. 中国农业大学学报，2009，（2）：16-21.

牛巍，侯彩云，祝晓芳，等. 三氯乙酸沉淀法与硫酸铜沉淀法在液态奶蛋白质检测中适用性研究[J]. 中国乳品工业，2008，36（9）：59-61.

刘媛，谢孟峡，龙梅等.FMOC-CL为柱前衍生化试剂对氨基酸PR-HPLC定量分析方法的研究[J].现代仪器，1999，（6）:14-17.

刘惠文.柱前和柱后衍生高效液相色谱分析氨基酸方法进展与评述[J].氨基酸和生物资源，1995,17（2）:50-55.

郭立安，阎哲，张晓楠，等.三氯乙酸对蛋白质结构稳定性的影响［J］.第四军医大学学报，2001，22( 22) :40.

任国谱, 李忠海, 彭美纯, 等. 奶粉中低聚肽质量浓度测定方法[J].中国乳品工业, 2007, 35(10): 47-49.

JOSHI S, YADAV S, DESAI A J. Application of response-surface methodology to evaluate the optimum medium components for the enhanced production of lichenysin by Bacillus licheniformis R2[J]. Biochemical Engineering Journal, 2008, 41(2): 122-127.

冯培勇, 钟旭生, 杨立红, 等. 利用响应面法优化茶薪菇产纤维素酶的发酵条件[J]. 食品科学, 2009, 30(7): 162-165.

吴竹青, 陈景, 黄群, 等. 响应面法优化雪莲果酒发酵工艺[J]. 食品科学, 2010, 31(23): 182-187.

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