Optimizing Conditions for Alcalase-Catalyzed Hydrolysis of Grass Carp Protein by Response Surface Methodology

doi:10.7506/spkx1002-6630-201404006

Abstract

Abstract:

This study aimed to optimize the conditions for enzymatic hydrolysis of grass carp proteins. Alcalase was used
to hydrolyze grass carp proteins. The effects of enzyme/substrate ratio, pH value, temperature, material/liquid ratio and
hydrolysis time on the degree of hydrolysis were analyzed. Box-Behnken design (BBD) and response surface methodology
(RSM) were adopted to obtain the optimum hydrolysis conditions on the basis of results of single-factor experiments. The
degree of hydrolysis of 23.46% was obtained under the optimum hydrolysis conditions of 1.8%, 55 ℃, 1:15, 180 min and 9.0
for enzyme/substrate ratio, temperature, material/liquid ratio, hydrolysis time, and pH, respectively.

Key words: grass carp protein, enzymatic hydrolysis, response surface methodology

CLC Number:

TS254.4

SHI Ling, ZHAO Li*, YUAN Mei-lan, SU Wei, LIU Hua, CHEN Li-li, WEN Hui-fang. Optimizing Conditions for Alcalase-Catalyzed Hydrolysis of Grass Carp Protein by Response Surface Methodology[J]. FOOD SCIENCE, 2014, 35(4): 26-29.

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