Quadratic Regression Central Composite Method for the Optimization of Enzymatic Extraction of Vanillin from Vanilla (Vanilla planifolia Andrews) Pods with Exogenous

doi:10.7506/spkx1002-6630-201318004

FOOD SCIENCE

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Quadratic Regression Central Composite Method for the Optimization of Enzymatic Extraction of Vanillin from Vanilla (Vanilla planifolia Andrews) Pods with Exogenous

MO Li-mei，ZHANG Yan-jun，GU Feng-lin，XU Fei，LU Min-quan

1. College of Food Science, Hainan University, Haikou 570228, China；
2. Institute of Spice and Beverage Research, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, China；
3. Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture, Wanning 571533, China

Online:2013-09-25 Published:2013-09-27

Abstract

Abstract:

This study was conceived to develop an optimized procedure for enzymatic extraction of vanillin from vanilla green pods
with exogenous cellulase. For this, quadratic regression central composite design combined with response surface methodology was
employed to investigate the effects of enzymatic hydrolysis temperature, cellulase dosage and enzymatic hydrolysis time on vanillin
content. The results showed that the optimum conditions of vanilla green pods, enzymatic hydrolysis temperature, cellulase and
enzymatic hydrolysis time were 10 g, 53 ℃, 26.9 mg and 16 h, respectively. The maximum vanillin content under these conditions
was 2.18% compared to 1.97% obtained with the conventional method of fermentation and subsequent ethanol reflux extraction.

Key words: vanillin, cellulase, condition optimization

MO Li-mei，ZHANG Yan-jun，GU Feng-lin，XU Fei，LU Min-quan. Quadratic Regression Central Composite Method for the Optimization of Enzymatic Extraction of Vanillin from Vanilla (Vanilla planifolia Andrews) Pods with Exogenous[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201318004.

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Quadratic Regression Central Composite Method for the Optimization of Enzymatic Extraction of Vanillin from Vanilla (Vanilla planifolia Andrews) Pods with Exogenous

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