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Development of Lateral Flow Assay for Melamine Detection Based on Colloidal Gold

XIA Jun,LAI Wei-hua,TU Ji-ping,XU Guo-mao,ZHANG Xin-yu,FENG Rong-hua,ZHONG Ping-hai,HE Xiao-li   

  1. 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
    2. Jiangxi Province Institude of Veterinary Drug and Feed Control, Nanchang 330029, China;
    3. Jiangxi Zodolabs Biological Engineering Co. Ltd., Nanchang 330047, China
  • Online:2013-10-25 Published:2013-09-28

Abstract:

A method for rapid detection of melamine by colloidal gold strip was introduced. Four steps were involved in the
process of preparing the strip, including production of colloidal gold, manufacture of immunogold, installation and analysis
of the strip. With the strip, 10 μg/L of melamine was detected in less than 10 min. Fresh milk could be detected directly without
pretreatment, so it provided a simple, accurate and rapid method for on-site testing of melamine.

Key words: colloidal gold, melamine, immunochromatography, detection