Rapid Determination of Enrofloxacin in Milk by Fluorescence Spectroscopy Combined with Chemometrics

Abstract

Abstract:

Though there are many analytical methods available to determine antibiotic residues in milk, most of them are
rather complicated. A rapid screening method for enrofloxacin in milk was proposed by fluorescence spectroscopy combined
with chemometrics. The fluorescence spectra of milk samples spiked with different concentrations of enrofloaxcin (ENRO)
were recorded and partial least square discriminate analysis (PLSDA) and partial least square (PLS) regression methods were
conducted for use as calibration and prediction models. The results showed that the PLS model could determine the concentration
of ENRO in milk, and the recovery rate for milk samples was in the range of 96% to 111.4%. The PLSDA model
could accurately identify whether ENRO levels in milk samples exceeded the maximum residue limit with good repeatability.
Therefore the established models are useful for rapid determination of ENRO residues in milk.

Key words: milk, enrofloxacin, fluorescence, chemometrics, rapid determination

CLC Number:

TS252.7

LIU Xiao-ming，FENG Shi-yun，ZHOU Ying-zhe，TIAN Feng-wei，ZHAO Jian-xin，ZHANG Hao，CHEN Wei. Rapid Determination of Enrofloxacin in Milk by Fluorescence Spectroscopy Combined with Chemometrics[J]. FOOD SCIENCE, 2013, 34(22): 111-114.

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