Effect of Fumonisin B1 on the mRNA Expressions of Cell Cycle-related Genes in Human Normal Esophageal Epithelial Cells

doi:10.7506/spkx1002-6630-201323063

Abstract

Abstract:

Epidemiological studies have shown that fumonisin B1 (FB1), a fungal toxin, may be associated with esophageal
cancer, but the laboratory evidence is still limited. In this study, human normal esophageal epithelial cells were isolated and
identified in vitro. The influence of FB1 on the mRNA expression of cell cycle-related genes including Cyclin D1, Cyclin E, P16,
P21, and P27 was determined by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that FB1
played an important role in regulating the expression of Cyclin D1, Cyclin E, P16, P21, and P27 genes in normal human
esophageal epithelial cells at the transcriptional level, leading to high mRNA expression of Cyclin D1 but low mRNA
expression of Cyclin E, P16, P21, and P27 genes. The abnormal expression of cell cycle-related genes is closely related with
cancer. This in vitro study has further confirmed that FB1 is related with the occurrence of human esophageal cancer.

Key words: fumonisin B1, human normal esophageal epithelial cells, cell cycle, gene expression

CLC Number:

R155.5

WANG Shao-kang，WANG Ting-ting，HUANG Gui-ling，SHI Ruo-fu，YANG Li-gang，DONG Shu-nan，SUN Gui-ju*. Effect of Fumonisin B1 on the mRNA Expressions of Cell Cycle-related Genes in Human Normal Esophageal Epithelial Cells[J]. FOOD SCIENCE, 2013, 34(23): 313-316.

References

[1] Gelderblom W C, Rheeder J P, Leggott N, et al. Fumonisin contamination of a corn sample associated with the induction of hepatocarcinogenesis in rats-role of dietary deficiencies[J]. Food Chem Toxicol, 2004, 42(3): 471-479

[2] Marasas W F, Jaskiewicz K, Venter F S, et al. Fusarium moniliforme contamination of maize in oesophageal cancer areas in Transkei[J]. S Afr Med J, 1988, 74(3): 110-114

[3] Yoshizawa T, Yamashita A, Luo Y. Fumonisin occurrence in corn from high- and low-risk areas for human esophageal cancer in China[J]. Appl Environ Microbiol, 1994, 60(5): 1626-1629

[4] Chu F S, Li G Y. Simultaneous occurrence of fumonisin B1 and other mycotoxins in moldy corn collected from the People's Republic of China in regions with high incidences of esophageal cancer[J]. Appl Environ Microbiol, 1994, 60(3): 847-852

[5] Sun G, Wang S, Hu X, et al. Fumonisin B1 contamination of home-grown corn in high-risk areas for esophageal and liver cancer in China[J]. Food Addit Contam, 2007, 24(2): 181-185

[6] 邱茂锋，刘秀梅，王玉华，等. 某食管癌高发区人群伏马菌素摄入量及尿二氢神经鞘氨醇/神经鞘氨醇比值的调查[J]. 卫生研究, 2001, 30(6): 365-367

[7] Wang H, Wei H, Ma J, et al. The fumonisin B1 content in corn from North China, a high-risk area of esophageal cancer[J]. J Environ Pathol Toxicol Oncol, 2000, 19(1-2): 139-141

[8] Alizadeh A M, Rohandel G, Roudbarmohammadi S, et al. Fumonisin B1 contamination of cereals and risk of esophageal cancer in a high risk area in northeastern Iran[J]. Asian Pac J Cancer Prev, 2012, 13(6): 2625-2628

[9] Wang S K, Liu S, Yang L G, et al. Effect of fumonisin B1 on the cell cycle of normal human liver cells[J]. Mol Med Rep, 2013, 7(6): 1970-1976

[10] Bondy G S, Barker M G, Lombaert G A, et al. A comparison of clinical, histopathological and cell-cycle markers in rats receiving the fungal toxins fumonisin B1 or fumonisin B2 by intraperitoneal injection[J]. Food Chem Toxicol, 2000, 38(10): 873-886

[11] Ho Y S, Chen C H, Wang Y J, et al. Tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) induces cell proliferation in normal human bronchial epithelial cells through NFkappaB activation and cyclin D1 up-regulation[J]. Toxicol Appl Pharmacol, 2005, 205(2): 133-148

[12] Hwang H C, Clurman B E. Cyclin E in normal and neoplastic cell cycles[J]. Oncogene, 2005, 24(17): 2776-2786

[13] Nanos-Webb A, Jabbour N A, Multani A S, et al. Targeting low molecular weight cyclin E (LMW-E) in breast cancer[J]. Breast Cancer Res Treat, 2012, 132(2): 575-588

[14] Risch H A. Cyclin E overexpression relates to ovarian cancer histology but not to risk factors[J]. Cancer Epidemiol Biomarkers Prev, 2008, 17(7): 1841-1842

[15] O'Regan E M, Toner M E, Finn S P, et al. p16(INK4A) genetic and epigenetic profiles differ in relation to age and site in head and neck squamous cell carcinomas[J]. Hum Pathol, 2008, 39(3): 452-458

[16] Zhang Y, Dickman M B, Jones C. The mycotoxin fumonisin B1 transcriptionally activates the p21 promoter through a cis-acting element containing two Sp1 binding sites[J]. J Biol Chem, 1999, 274(18): 12367-12371

[17] Zhang W G, Wu Q M, Yu J P, et al. Adenovirus expressing p27kip1 suppresses growth of established esophageal carcinoma xenografts[J]. World J Gastroenterol, 2005, 11(42): 6582-6586