Abstract:

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) method was developed
for the simultaneous determination of seven drug residues including 6-benzylaminopurine, para-chlorophenoxyacetic acid,
2,4-D, gibberellin A3, carbendazim, procymidone, and enrofloxacin in bean sprouts. The seven compounds from samples
were simultaneously extracted with acetonitrile and analyzed by HPLC-MS-MS. The calibration curves showed good
linearity in the range of 0.01–10 μg/mL for 6-benzylaminopurine, para-chlorophenoxyacetic acid, 10-benzylaminopurine,
gibberellin A3, and procymidone, and in the range of 0.01–1 μg/mL for carbendazim and enrofloxacin with correlation
coefficients more than or equal to 0.987. The recoveries of these drug residues at three spiked levels (0.002, 0.2 and
0.5 mg/kg) ranged from 60.31% to 108.51%, with relative standard deviation (RSDs) between 1.03% and 13.10% and
method detection limit (MDL) between 0.010 5 and 0.125 μg/kg.

Key words: high performance liquid chromatography tandem mass spectrometry (HPLC-MS-MS), bean sprouts;
6-benzylaminopurine, para-chlorophenoxyacetic acid, 2,4-D, gibberellin A3, carbendazim , procymidone, enrofloxacin