Abstract:

The effects of other alcohols on the Lipozyme RM IM-catalyzed ethanolysis of soy phosphatidylcholine (PC)
for the preparation of lysophosphatidylcholine (LPC) were studied and the ethanolysis conditions were optimized. The
results showed that n-butanol had no significant synergistic or inhibitory effect on the ethanolysis of PC, while tert-butanol
displayed an inhibitory effect, and 1,2-propylene glycol and glycerol exhibited a significant synergistic effect. Glycerol
was chosen as the synergistic agent for PC ethanolysis. The optimal conditions for the ethanolysis catalyzed by Lipozyme
RM IM in solvent-free system were obtained by response surface methodology as follows: lipase dosage, 15% (based on
the mass of PC); temperature, 28 ℃; ratio of water to ethanol solution, 8% (V/V); ratio of glycerol to ethanol solution, 10%
(V/V); substrate concentration, 1.49 g/mL (PC/ethanol solution ratio, m/V), and reaction time, 11 h. Under these conditions,
the conversion rate of LPC was 98.2%. Glycerol exhibited a significant synergistic effect on the ethanolysis of PC.

Key words: phosphatidylcholine, Lipozyme RM IM, ethanolysis, lysophosphatidylcholine