FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (7): 114-117.doi: 10.7506/spkx1002-6630-200907026

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Determination of Enzymatic Hydrolysis Process of Egg Yolk Phospholipid

WANG Ying-xin CHI Yu-jie*   

  1. (College of Food, Northeast Agricultural University, Harbin 150030, China)
  • Received:2008-05-27 Revised:2008-11-24 Online:2009-04-01 Published:2010-12-29
  • Contact: CHI Yu-jie* E-mail:yjchi@126.com;chiyujie@hotmail.com

Abstract:

The emulsifying property of egg yolk decreases sharply in high temperature processing of food. Enzymatic method can improve the emulsifying property and thermal stability of egg yolk. In order to monitor the hydrolysis reaction, the hydrolysis products, free fatty acids (FFA) were determined by improved Dole method. Moreover, the correlation between the improved Dole method and high-performance liquid chromatography (HPLC) in monitoring the hydrolysis process was also investigated. The results showed that there is a good linear relationship between the generation of FFA determined by Dole method and the reduction of phosphatidylcholine determined by HPLC with a correlation coefficient of 0.999. And the feasibility of Dole method was verified for monitoring the reaction process. In conclusion, the Dole method is a fast, simple and accurate way.

Key words: egg yolk, phospholipase A2, Dole method, free fatty acids (FFA), HPLC, phosphatidylcholine

CLC Number: