Abstract:

This report describes the selection and optimization of experimental conditions for cellulase-assisted extraction
of total flavonoids from Allium mongolicum Regel using a combination of single factor experiment and response surface
methodology. It was demonstrated that the optimal extraction parameters were determined to be hydrolysis at 39 ℃ for 4 h
with an initial solvent pH of 4.3, leading to an extraction yield of 5.48 mg/g. Further this study found that the extracted
flavonoids had good total reduction capability and scavenging activities against DPPH and hydroxyl radicals, and exhibited
strong inhibition against Salmonella. However, the inhibitory effect against E. coli and Staphylococcus aureus was weak,
and no inhibition against Pseudomonas aeruginosa was observed.

Key words: Allium mongolicum Regel, flavonoids, response surface methodology, antioxidant activity in vitro, antibacterial activity in vitro