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Simultaneous Determination of 195 Pesticide Residues in Vegetables Using Solid Phase Dispersive xtraction-Gas Chromatography-Tandem Mass Spectrometry

LI Rong1, LU Jun-wen1, YANG Fang1, ZHANG Peng-jie1, LI Yong-jun2, XIE Jian-jun3, CHU Da-ke4   

  1. 1. Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528403, China;
    2. Zhongshan Quality Supervision and Inspection Institute of Agricultural Products, Zhongshan 528400, China;
    3. Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China;
    4. Guangdong Pharmaceutical University, Zhongshan 528400, China
  • Online:2014-12-25 Published:2014-12-29

Abstract:

Objective: To develop a method for the determination of 195 pesticide residues in vegetables using gas
chromatography with tandem mass spectrometry (GC-MS-MS) under a selected reaction monitoring (SRM) mode. Methods:
The target compounds were extracted from samples with ethyl acetate and the extract was cleaned up by off-line solid phase
dispersive extraction technique. The analytes were quantified by an internal standard method. Results: In the linear range
(0.01–1.00 mg/L), the correlation coefficient for each pesticide was higher than 0.99. The average recoveries at three spiked
levels (10, 20 and 100 μg/kg) varied from 37.6% to 136.7% with relative standard deviations (RSDs) between 0.2% and 15.3%.
The limits of quantification (LOQ) (signal/noise ratio = 10) were 1.9–25.5 μg/kg. Conclusion: The method is rapid, sensitive,
accurate and high throughput, without matrix interference and suitable for the analysis of 195 pesticide residues in vegetables.

Key words: solid phase dispersive extraction, gas chromatography-tandem mass spectrometry (GC-MS-MS), pesticide residues, vegetable

CLC Number: