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Microwave Extraction and Purification of Flavonoids from Illicium verum Leaves

LIU Tao, LI Rong*, ZHANG Lujie, JIANG Zitao   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce,
    Tianjin 300134, China
  • Online:2015-01-25 Published:2015-01-16
  • Contact: LI Rong
  • Supported by:

    天津市自然科学基金重点资助项目(12JCZDJC34100)

Abstract:

The conditions for microwave-assisted extraction of flavonoids from Illicium verum leaves were optimized by the
combined use of Plackett-Burman design and response surface methodology (RSM) as follows: ethanol concentration, 67%;
ratio of solvent to solid, 26:1; extraction temperature, 75 ℃; initial microwave power, 500 W; and extraction time, 8 min.
The yield of total flavonoids was 6.97% under these conditions. The flavonoids from I. verum leaves were purified by D101
macroporous resin in comparison with preparative chromatography. The results indicated that preparative chromatography
is not only an efficient and rapid method for the purification of flavonoids extract, but also can preliminarily separate
flavonoids.

Key words: Illicium verum leaves, response surface, macroporous resins, preparative chromatography

CLC Number: