Abstract:

The purification of flavonoids from Cordyceps militaris using macroporous resin was optimized by investigating
several process parameters including resin type, sample volume, eluent pH, concentration and volume, and repeated use
of the selected resin. Static adsorption and desorption, and kinetic studies of static adsorption demonstrated that AB-8 was
selected as the better adsorbent with higher adsorption rate and adsorption capacity per unit mass for Cordyceps militaris
flavonoids compared with D-101, NKA-9 and NKA-Ⅱ. As determined by optimization experiments, the recovery of
flavonoids was more than 65% with a purity of over 17% when 40.0 mL of the sample (47.536 mg of flavonoids) was loaded
onto a 100-mL column at a flow rate of 2 BV/h of AB-8 followed by elution with 500 mL of 85% ethanol acidified to pH 5
also at flow rate of 2 BV/h and the resin was used to repeat the process once more.

Key words: Cordyceps militaris, flavonoids, macroporous resins, purification