Abstract:

The composition of a sugarcane molasses-based medium used to culture Hanseniaspora uvarum CM2 was
optimized for enhanced biomass production. The molasses was pretreated by boiling or adding phosphoric acid, sulfuric
acid, potassium ferrocyanide and activated carbon, respectively. Comparing their impacts on the growth of the antagonistic
yeast CM2, sulfuric acid addition was considered the method of choice. Molasses concentration, the type and concentration
of nitrogen source, initial pH, phosphate, magnesium sulfate, and sodium chloride were studied for their influence on the
biomass production of CM2 by single factor design. Plackett-Burman design and response surface methodology were used
to optimize the medium components as pretreated molasses 78.9 g/L, yeast powder 2.2 g/L and initial pH 6.0. The optimal
medium could result in an 83.1% enhancement in the biomass production of CM2.

Key words: Hanseniaspora uvarum, sugarcane molasses, Plackett-Burman experimental design, medium, biomass