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Optimization of Protein Extraction from Selenium-Enriched Soybean and Determination of Selenomethionine by Liquid Chromatography Coupled with Mass Spectrometry

TIE Mei1, LI Baorui1, XING Zhiqiang2, HAN Jie1, GAO Yu1, ZHANG Anning1, ZHUANG Xiaohong1,*   

  1. 1. College of Environmental Sciences, Liaoning University, Shenyang 110036, China;
    2. College of Chemistry, Liaoning University, Shenyang 110036, China
  • Online:2015-04-25 Published:2015-04-28
  • Contact: ZHUANG Xiaohong

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Abstract:

Based on single factor experiments, the extraction process for selenium-enriched soybean protein was optimized
through Box-Behnken central composite design and response surface methodology. Selenoamino acids were obtained from soluble
protein under the optimized extraction conditions. Selenomethionine was qualitatively and quantitatively analyzed by liquid
chromatography coupled with mass spectrometry. The optimal conditions for extraction were determined as the follows: liquid/
material ratio, 11:1; extraction temperature, 44 ℃; and extraction time, 96 min. The yield of soluble selenium-containing protein
under these conditions was 76.03%. Analysis of soluble proteins from four soybeans enriched with four different levels of selenium
indicated that the content of selenium in selenomethionine accounted for 28.46% of the total selenium. Thus, other selenoamino
acids also existed besides selenomethionine. Soybeans were able to not only absorb and accumulate selenium, but also to transform
inorganic selenium into organic selenium compounds with higher bioavailability and less toxicity. This study provides a scientific
approach for developing selenium-enriched functional organic food based on soybeans.

Key words: selenium-enriched soybean, liquid chromatography coupled with mass spectrometry, selenomethionine

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