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Optimization of Extraction Process of Yeast Metallothionein by Aqueous Two-Phase System

WANG Yue1, ZHANG Dongjie1,*, WANG Ying1,2,*, LIANG Xiaoyue1, XU Bingzheng1   

  1. 1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
    2. National Coarse Cereals Engineering Research Center, Daqing 163319, China
  • Online:2015-05-25 Published:2015-05-08
  • Contact: ZHANG Dongjie1, WANG Ying

Abstract:

In this study, an aqueous two-phase system consisting of polyethylene glycol (PEG) and ammonium sulfate was
used to extract copper-induced metallothionein (MT) from a mutant strain of Saccharomyces cerevisiae. Response surface
methodology was employed to optimize three factors namely PEG concentration, (NH4)2SO4 concentration and pH that
influence extraction efficiency using the software Design-Expert 8.0.6. The optimal aqueous two-phase system for MT
extraction was determined to contain 22.5% PEG 2 000, 17.46% (NH4)2SO4 at pH 5.98. Under these optimal conditions, the
metallothionein was mainly distributed in the upper phase with an extraction rate of 81.69%. Therefore, this method can
provide a new, simple, convenient, economical and highly efficient strategy for the extraction of metallothionein from yeast.

Key words: metallothionein, aqueous two-phase system extraction, response surface methodology, distribution coefficient;extraction rate

CLC Number: