Analysis of Monosaccharide Composition of Polysaccharide from Nostoc sphaeroides Kützing

doi:10.7506/spkx1002-6630-201518016

Abstract

Abstract:

Two analytical methods, namely high performance liquid chromatography (HPLC) and HPLC-mass spectrometry
(HPLC-MS) using precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone were established for analyzing the
monosaccharide composition of polysaccharide from Nostoc sphaeroides Kützing. Consistent results were obtained using
both methods. The standard curve developed using monosaccharide standards by HPLC exhibited a correlation coefficient
of greater than 0.995. According to the standard curves, the polysaccharide in N. sphaeroides was composed of manose,
glucuronic acid, glucose, galactose and arabinose at a molar ratio of 1:1.12:3.24:1.72:1.21.

Key words: Nostoc sphaeroides Kützing polysaccharide, monosaccharide, precolumn derivatization, HPLC, MS

CLC Number:

TS201.2

MO Kaiju, ZHAO Na, ZHU Zhaowu, TIAN Panpan, CHENG Chao. Analysis of Monosaccharide Composition of Polysaccharide from Nostoc sphaeroides Kützing[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201518016.

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Analysis of Monosaccharide Composition of Polysaccharide from Nostoc sphaeroides Kützing

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