Abstract:

In this work, Raman spectroscopy was employed to directly monitor the changes in the characteristic peaks of
the unsaturated double bonds of Ganoderma lucidum spore oil during oxidative rancidity and the relationship between the
peak intensity and oxidative rancidity was discussed. Raman scattering intensity decreased with increasing acid value and
peroxide value and a linear correlation between acid value and the area of the characteristic peaks was determined. The
kinetic parameters for the oxidative rancidity of Ganoderma lucidum spore oil were also determined. As a result, a new
and fast approach for the detection of oxidative rancidity in Ganoderma lucidum spore oil by Raman spectroscopy was
established. This method was further validated and used to measure the kinetic parameters of oxidative rancidity and screen
antioxidants for Ganoderma lucidum spore oil.

Key words: Raman spectroscopy, Ganoderma lucidum spore oil, oxidative rancidity, rapid detection