Preparation of Blended Calcium Powders from Shrimp Shell and Evaluation of Its Calcium Absorption in Rats

doi:10.7506/spkx1002-6630-201614001

Abstract

Abstract:

Purpose: To optimize the preparation of blended calcium powders from shrimp shell and to evaluate its calcium
absorption in rats. Methods: The extraction of blended calcium powders from shrimp shell (Penaeus vannamei) by the
combined use of trypsin and citric acid was investigated. Furthermore, the effects of calcium powder on growth, calcium
absorption in rats was studied by establishing calcium deficiency model. Results: The optimized preparation conditions
were determined as follows: initial hydrolysis with 2.5 × 103 U/g of trypsin at pH 8.0 and 45 ℃ for 1.5 h followed by
treatment with 20 mL of 0.35 mol/L citric acid for 1.5 h. Under these conditions, the rate of proteolysis was 57.24%, and the
dissolution rate of calcium was 85.78%. Furthermore, the calcium power contained 19.6% calcium and 18.3% total amino
acids, which was a hazel powder with a delicate texture and without off-flavor. Animal experiments showed that the calcium
powder prepared from shrimp shell significantly improved calcium absorption in rats (P < 0.05), and that gavage dose of
66.5 mg/(kg·d) (calculate based on calcium content and the body weight of rats) could improve the contents of calcium and
phosphorous in serum. Conclusion: Trypsin combined with citric acid offers a mild and effective way to extract blended
calcium powder from shrimp shell, which exhibits better absorption than of CaCO3 with the same calcium content.

Key words: shrimp shell, blended calcium powders, trypsin, citric acid.

CLC Number:

TS254.9

ZHANG Wenjing, CHEN Shunsheng. Preparation of Blended Calcium Powders from Shrimp Shell and Evaluation of Its Calcium Absorption in Rats[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201614001.

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