Combined Use of ELISA and UPLC-MS/MS for Detection of Zearalenone from Grains

doi:10.7506/spkx1002-6630-201616037

Abstract

Abstract:

An enzyme-linked immunosorbent assay (ELISA) coupled to ultra performance liquid chromatography-tandem
mass spectrometry (UPLC-MS/MS) method was developed for the determination of zearalenone in corn samples. Samples
were extracted with acetonitrile-water-acetic acid (70:29:1, V/V) and then the extract was determined by ELISA and UPLCMS/
MS. In the study, special attention was given to the stability of the system and the matrix effects frequently observed in
the process. The recovery of UPLC-MS/MS with and without sample purification was evaluated as well. Proper approaches
for dealing with these problems were obtained. This analysis method proved easy, fast and suitable for the determination of
zearalenone in bulk grain samples.

Key words: zearalenone, ELISA, UPLC-MS/MS, grain, detection

CLC Number:

TS207.5

LI Yan, PEI Shichun, WANG Yan, GAO Jianwei, ZHEN Yuping, MA Mingxin, ZHANG Pin, XIE Tiezhi. Combined Use of ELISA and UPLC-MS/MS for Detection of Zearalenone from Grains[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201616037.

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Combined Use of ELISA and UPLC-MS/MS for Detection of Zearalenone from Grains

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