Abstract:

In this study, an optimized method using high performance liquid chromatography-evaporative light scattering
detector (HPLC-ELSD) was developed for detecting trehalose extracted from three edible fungi including Auricularia
auricula-judae, Pleurotus ostreatus, and Pleurotus citrinopileatus Sing., which were cultivated on mixed substrates
containing assava (Manihot esculenta) stalk. The relationship between trehalose content and cassava stalk proportion was
explored. The results indicated that the trehalose in edible fungi could be completely extracted in 80 ℃ water bath in 60
minutes, and the maximum yield of trehalose could reach 190.5 mg/g. Under the optimized chromatographic conditions,
good separation of trehalose and maximum contents of trehalose were obtained from edible fungi cultivated on a substrate
with 31.2% cassava stalk, which were 61.5, 250.3 and 15.0 mg/g for Auricularia auricula-judae, Pleurotus ostreatus,
and Pleurotus citrinopileatus Sing., respectively. This study has demonstrated that the trehalose content of edible fungi
can be accurately and conveniently determined by HPLC-ELSD. In addition, the trehalose contents of A. auricula-judae,
P. ostreatus and P. citrinopileatus can be significantly improved by cultivating them on a substrate with a proper ratio of
cassava stalk.

Key words: cassava stalk, edible fungi, trehalose, high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD)