Preparation and Activity Determination of Colloidal Gold Probe Based on Anti-Listeria monocytogenes Single Chain
Fv Antibody Fragment

doi:10.7506/spkx1002-6630-201704049

Abstract

Abstract: In this study, an anti-Listeria monocytogenes (Lm) single chain Fv antibody fragment was expressed in Escherichia coli. After purification, the antibody fragment was used to prepare a colloidal gold probe with colloidal gold as a tracer and the preparation process was optimized. The activity of the probe was determined by using an immunofiltration assay. The results showed that the colloidal gold probe exhibited high specificity. Positive samples could be directly identified by color development using the probe. The detection limit (LOD) for Lm was approximately 107 CFU/mL and the whole process of immunofiltration assay took approximately 10 min. The method developed is simple, rapid and accurate, and it could be applied for Lm detection in food samples.

Key words: Listeria monocytogenes, single chain Fv antibody fragment, colloidal gold, immunofiltration assay

CLC Number:

TS207.3

LIU Aiping, YE Zixiong, MA Yu, ZHANG Zhouli, XIONG Qing, LI Wenli, SUN Haiqin, LI Cheng. Preparation and Activity Determination of Colloidal Gold Probe Based on Anti-Listeria monocytogenes Single Chain Fv Antibody Fragment[J]. FOOD SCIENCE, 2017, 38(4): 301-305.

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Preparation and Activity Determination of Colloidal Gold Probe Based on Anti-Listeria monocytogenes Single Chain Fv Antibody Fragment

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