Immobilization of Ganoderma lucidum in Sodium Alginate Beads and Effect on Extracellular Triterpene Production

doi:10.7506/spkx1002-6630-201205049

Abstract

Abstract: In this study, Ganoderma lucidum were immobilized by sodium alginate and calcium chloride. Immobilization conditions of Ganoderma lucidum using entrapment method were studied. The effects of immobilization conditions on triterpenes yield were also evaluated. The results showed the optimal conditions of immobilization of Ganoderma lucidum by sodium alginate were as follows: 4.5% of sodium alginate, 2.2% of calcium chloride, nature pH, 40 immobilized beads, 28 ℃, 110 r/min, cultured 12 days, the highest triterpenes yield was (49.53±1.37)×10-2 mg/mL. Immobilized cells had better acid and alkali resistance, higher triterpenes yield after long time culture than non-immobilized cell. It can be recycled five times.

Key words: Ganoderma lucidum, triterpenoids, immobilization, sodium alginate, calcium chloride

CLC Number:

TS201.3

DONG Yu-wei，TANG Bu-wei，MIAO Jing-zhi，CAO Ze-hong. Immobilization of Ganoderma lucidum in Sodium Alginate Beads and Effect on Extracellular Triterpene Production[J]. FOOD SCIENCE, 2012, 33(5): 234-237.

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Immobilization of Ganoderma lucidum in Sodium Alginate Beads and Effect on Extracellular Triterpene Production

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