Antibacterial Activity and Mechanism of Bifidocin A against Staphylococcus aureus

doi:10.7506/spkx1002-6630-201717001

Abstract

Abstract: Bifidocin A, produced by Bifidobacterium animalis BB04, is a novel bacteriocin with antimicrobial activity against a wide range of foodborne bacteria. The objective of this study was to investigate the antibacterial activity and mechanism of action of bifidocin A against Staphylococcus aureus. The minimum inhibitory concentration (MIC) of bifidocin A for Staphylococcus aureus CVCC 26112 was 0.058 μg/mL. Time-kill assays showed that bifidocin A effectively inhibited the growth of Staphylococcus aureus CVCC 26112 in a time- and concentration-dependent manner. Results also suggested that bifidocin A exerted its anti-Staphylococcus aureus effect through the dissipation of the cytoplasmic membrane proton motive force (PMF), increased membrane permeability, and the formation of cell membrane pores, leading to the destruction of membrane integrity and ultimately complete disintegration of the cells.

Key words: bacteriocin, Bifidobacterium spp., Staphylococcus aureus, antibacterial activity, antibacterial mechanism

CLC Number:

TS201.3

LIU Guorong, GAO Yakun, WANG Xin, LIU Yujie, CAO Jilin, WANG Chengtao. Antibacterial Activity and Mechanism of Bifidocin A against Staphylococcus aureus[J]. FOOD SCIENCE, 2017, 38(17): 1-7.

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Antibacterial Activity and Mechanism of Bifidocin A against Staphylococcus aureus

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