FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (17): 238-243.doi: 10.7506/spkx1002-6630-201717039

• Nutrition & Hygiene • Previous Articles     Next Articles

Preventive Effect of Lactobacillus plantarum YS-1 on Activated Carbon-Induced Constipation in Mice

YI Ruokun, QIAN Yu, WANG Qiang, MU Jianfei, ZHAO Xin*   

  1. Chongqing Collaborative Innovation Center for Functional Food, Chongqing Engineering Research Center of Functional Food,Chongqing Engineering Laboratory for Research and Development of Functional Food,Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
  • Online:2017-09-15 Published:2017-09-12

Abstract: The aim of this study was to determine the effect of Lactobacillus plantarum YS-1 (LP-YS1) on activated carboninduced constipation in Kunming mice. The experimental results showed that anti-gastric acid and bile salt activities of LP-YS1 were stronger than that of Lactobacillus bulgaricus. LP-YS1 could inhibit the decrease in body weight, fecal weight, fecal pellet number and fecal moisture content caused by constipation in mice. Meanwhile, LP-YS1 could raise gastrointestinal (GI) transit rate and reduce the time to the first black stool defecation. LP-YS1 also could increase serum motilin (MTL), endothelin (ET), acetylcholinesterase (AChE), substance P (SP), and vasoactive intestinal peptide (VIP) levels and decrease somatostatin (SS) level in constipated mice. reverse transcription polymerase chain reaction experiments revealed that LP-YS1 could raise c-Kit, stem cell factor (SCF) and glial cellline-derived neurotrophic factor (GDNF) mRNA expression and reduce transient receptor potential vanilloid 1 (TRPV1) and nitric oxide synthase (NOS) mRNA expression in the small intestine of constipated mice. High concentration of LP-YS1 had a better effect. Based on these results, LP-YS1 could effectively inhibit constipation.

Key words: Lactobacillus plantarum, activated carbon, constipation, motilin, endothelin, acetylcholinesterase

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