Separation, Purification and Monosaccharide Composition of Polysaccharides from the Ink of Sthenoteuthis oualaniensis in South China Sea

doi:10.7506/spkx1002-6630-201724019

Abstract

Abstract: Objective: To isolate and purify polysaccharides from the ink of Sthenoteuthis oualaniensis in the South China Sea, and to analyze the monosaccharide composition of the purified polysaccharides. Methods: Crude polysaccharides were isolated through water extraction followed by alcohol precipitation and passed through a solid phase extraction column to remove the pigment. The polysaccharides were fractionated and further purified into homogeneity by sequential chromatographies on DEAE-52 ion exchange and Sephacryl HR-300 columns. The monosaccharide composition was analyzed by ultra performance liquid chromatography (UPLC) after 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization. Results: Three purified polysaccharides designated as SIP1, SIP2, and SIP3 were obtained. SIP1 was composed of D-mannose, D-glucose, and D-galactose while SIP2 and SIP3 were composed of D-mannose, rat sugar, D-glucose, D-galactose and L-(-)-fucose in different ratios.

Key words: sepia ink polysaccharides, separation and purification, monosaccharide analysis

CLC Number:

TS254.4

HUANG Hui, YANG Lizhi, YANG Xianqing, LI Laihao, HAO Shuxian, WEI Ya, WANG Jinxu. Separation, Purification and Monosaccharide Composition of Polysaccharides from the Ink of Sthenoteuthis oualaniensis in South China Sea[J]. FOOD SCIENCE, 2017, 38(24): 118-123.

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Separation, Purification and Monosaccharide Composition of Polysaccharides from the Ink of Sthenoteuthis oualaniensis in South China Sea

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