Abstract: The uncertainty of measurement in the determination of iprodione residues in vegetables by high performance liquid chromatography (HPLC) was evaluated. According to the national metrology technical specifications JJF 1135-2005 (Evaluation of Uncertainty in Chemical Analysis Measurement) and JJF 1059.1-1999 (Evaluation and Expression of Uncertainty in Measurement), a mathematical model was established to evaluate the uncertainty for determining iprodione residues in vegetables. The sources of uncertainty that may be introduced were analyzed and each component of uncertainty was quantified and used to calculate combined uncertainty. The results showed that an expended uncertainty of 5.31 mg/kg (k = 2) was obtained for the determination of 0.40 mg/kg of iprodione in vegetables, and the preparation of standard solution, calibration curve fitting and measurement repeatability were the main sources of uncertainty.

Key words: high performance liquid chromatography, vegetables, iprodione, uncertainty