FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (23): 141-148.doi: 10.7506/spkx1002-6630-201723023

• Nutrition & Hygiene • Previous Articles     Next Articles

Anti-Aging Effect and Its Mechanism of Ethanol Extract from Sea Buckthorn Seed Meal in Caenorhabditis elegans

ZHANG Jiachan1, WANG Changtao1,2,*, LIU Yao1, SHI Xiuqin1, ZHAO Dan1, LI Meng1, WANG Chengtao2,3, SUN Baoguo2,3   

  1. 1. Beijing Key Laboratory of Plant Resources Research and Development, School of Science, Beijing Technology and Business University, Beijing 100048, China; 2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China; 3. Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China
  • Online:2017-12-15 Published:2017-12-07

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Abstract: In the present study, the anti-aging effect of sea buckthorn seed extracts (SBSE) obtained by ethanol extraction and macroporous resin purification was evaluated using Caenorhabditis elegans as a model organism and its underlying mechanism was discussed as well. The effect of different concentrations of SBSE was investigated on the lifespan, reproductive capability, heat and oxidative stress defense of C. elegans. In order to explore the anti-aging mechanism of SBSE at the gene level, the activity of antioxidant enzymes and the expression levels of related genes were determined. The results indicated that SBSE could significantly prolong the lifespan of C. elegans (P < 0.05), and reduce the accumulation of lipofuscin. Meanwhile, the number of C. elegans offsprings treated with 0.05 mg/mL of SBSE (246.40 ± 21.11) was significantly bigger than the control (205.80 ± 20.42) (P < 0.05). SBSE could significantly enhance the heat and oxidative stress defense (P < 0.05). Furthermore, SBSE increased the activity of superoxide dismutase, catalase and glutathione peroxidase, down-regulated the pro-aging gene expression levels of age-1 and daf-2, and up-regulated the anti-aging gene expression levels of sir-2.1. Therefore, SBSE possess an anti-aging effect by increasing the activity of antioxidant enzymes and changing the expression of aging-related genes.

Key words: sea buckthorn seed extracts, Caenorhabditis elegans, antioxidant enzymes, anti-aging

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