Structural Analysis and Anti-Pancreatic Lipase Activity of Flavonoids from Moringa oleifera Lam. Leaves

doi:10.7506/spkx1002-6630-201802006

Abstract

Abstract: The structure of flavonoids from Moringa oleifera Lam. leaves (FML) was analyzed. Meanwhile, the inhibitory effect of FML on pancreatic lipase was determined and the type of inhibition was investigated. The extraction yield of flavonoids reached (5.53 ± 0.11)% with microwave-assisted extraction using 70% ethanol as the extraction solvent. The obtained crude extract was purified by polyamide column chromatography and freeze-dried to obtain a powder. The total flavonoid content of the powder was (661.10 ± 9.20) mg/g. A total of 11 flavonoids were identified from FML by ultra performance liquid chromatography-quadrupole/electrostatic field orbital trap mass spectrometry (UPLC-Q-Orbitrap MS). The inhibitory activity of FML on pancreatic lipase was determined with p-nitrophenyl butyrate as substrate. The results showed that the purified FML had a strong inhibitory effect on pancreatic lipase, with an IC50 of 0.94 mg/mL, and the type of inhibition was determined as non-competitive inhibition by the Lineweaver-Burk double reciprocal plot method.

Key words: flavonoids from Moringa oleifera Lam. leaves, UPLC-Q-Orbitrap MS, pancreatic lipase, inhibitory effect

CLC Number:

TS201.4

WANG Yuan, ZHENG Wen, CAI Junjun, YUAN Tianqing, GONG Xingwen. Structural Analysis and Anti-Pancreatic Lipase Activity of Flavonoids from Moringa oleifera Lam. Leaves[J]. FOOD SCIENCE, 2018, 39(2): 31-37.

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Structural Analysis and Anti-Pancreatic Lipase Activity of Flavonoids from Moringa oleifera Lam. Leaves

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