FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (13): 151-155.doi: 10.7506/spkx1002-6630-201113033

• Bioengineering • Previous Articles     Next Articles

Purification of Specific Polyclonal Antibody against Salmonella typhimurium

NIU Rui-jiang,LAI Wei-hua*,XIONG Qi-rong,WEI Hua,XIONG Yong-hua   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Online:2011-07-15 Published:2011-07-02

Abstract: A highly specific anti-Salmonella typhimurium polyclonal antibody was prepared for immunological determination of Salmonella typhimurium. Anti-Salmonella typhimurium polyclonal antibody was generated in Japanese rabbits using formalin-inactivated Salmonella typhimurium as antigen and purified by octanoic acid-ammonium sulfate precipitation and magnetic bead-antigen coupling. The titer of the prepared highly pure antibody was 3.2 × 105; however, this antibody revealed serious cross-reaction with other bacteria. In this paper, the antibody was purified by miscellaneous bacterial antigens in a reverse adsorption mode, which could eliminate the cross reaction between the antibody and miscellaneous bacteria. Therefore, an effective purification method to obtain specific polyclonal antibody was established.

Key words: Salmonella typhimurium, polyclonal antibody, reverse adsorption, cross reactivity

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