Abstract:

To establish a PCR detection method for Salmonella typhimurium, the ompc gene was amplified by PCR using
the specific primers designed according to the sequence published in GenBank. The PCR reaction conditions were optimized
based on the sensitivity and specificity of this method. The results showed that the 470 bp DNA fragment was specifically
amplified from Salmonella typhimurium and no DNA fragment was obtained from other common pathogenic bacteria in
foods including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Shigella. The sensitivity of this PCR
method was 1 pg/μL. In conclusion, this experiment can lay a foundation for further exploration of rapid detection methods
for Salmonella typhimurium in foods.

Key words: Salmonella typhimurium, ompc gene, PCR, detection method