Construction of an in vitro Allergy Reaction Evaluation Method for Cow Milk and Its Substitutes Using Human Leukemia Cell Line KU812F

doi:10.7506/spkx1002-6630-201114042

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (14): 202-205.doi: 10.7506/spkx1002-6630-201114042

• Analysis & Detection • Previous Articles Next Articles

Construction of an in vitro Allergy Reaction Evaluation Method for Cow Milk and Its Substitutes Using Human Leukemia Cell Line KU812F

CONG Yan-jun1，REN Fa-zheng2,*

(1. Beijing Key Laboratory of Food Flavor Chemistry, Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China； 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)

Online:2011-07-25 Published:2011-06-18

Abstract

Abstract: This study aimed to construct an in vitro cellular assay for suspected allergenic components of cow milk and its substitutes using human leukemia cell line KU812F, an immature prebasophillic cell line based on their histamine release ability. KU812F was differentiated into basophils by incubation with serum from cow milk allergic people. The release of histamine from differentiated KU812F cells was monitored after sensitization with either cow milk or its substitutes. The results showed that this in vitro assay was highly reliable and reproducible in determining histamine release from KU812F cells after respective sensitization with cow milk and its products. Cow milk and its substitutes differed in their histamine release ability. Cow milk allergen induced histamine release in a dose-dependent manner. Specifically, a positive relationship was observed in a defined range of allergen dose, followed by a negative relationship after maximum histamine release.

Key words: KU812F, histamine, allergy, cow milk powder

CLC Number:

TS207.3

CONG Yan-jun，REN Fa-zheng. Construction of an in vitro Allergy Reaction Evaluation Method for Cow Milk and Its Substitutes Using Human Leukemia Cell Line KU812F[J]. FOOD SCIENCE, 2011, 32(14): 202-205.

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Construction of an in vitro Allergy Reaction Evaluation Method for Cow Milk and Its Substitutes Using Human Leukemia Cell Line KU812F

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