FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (16): 273-279.doi: 10.7506/spkx1002-6630-201816039

• Processing Technology • Previous Articles     Next Articles

Preparation of Dummy Molecularly Imprinted Polymers for Numb-Taste Components of Zanthoxylum bungeanum

LI Yao1, HE Yezi1, CHEN Guangjing1,2,3, KAN Jianquan1,2,3,*   

  1. (1. College of Food Science, Southwest University, Chongqing 400715, China; 2. Laboratory of Quality & Safety Risk Assessment for Agro-Products on Storage and Preservation (Chongqing), Ministry of Agriculture, Chongqing Key Laboratory of Produce Processing and Storage, Chongqing 400715, China; 3. Chinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, China)
  • Online:2018-08-25 Published:2018-08-17

Abstract: In this work, molecularly imprinted polymers (MIPs) with specific binding affinity for the numb-taste compounds of Zanthoxylum bungeanum were prepared by using structural analogs of alkyl amides (LM) as dummy template molecule. In order to optimize the preparation conditions, UV spectroscopy was applied to study the effect of intensity of interaction and molar ratio between dummy template and functional monomers. With ethylene glycol dimethacrylate (EDMA) as the cross-linker, acetonitrile as the pore-foaming agent, 2,2-azobisisobutyronitrile (AIBN) as the initiator, 16 MIPs were prepared by bulk polymerization and the best MIP was selected and used to separate numb-taste compounds from the extract of Z. bungeanum. The results showed that the selected MIP had specific recognition and selectivity for the numb-taste components and exhibited an adsorption capacity of (14.64 ± 0.80) mg/g as determined by high performance liquid chromatography (HPLC). The peak area percentage of numb-taste component increased by approximately 21.95% after adsorption by the MIP.

Key words: numb-taste components of Zanthoxylum bungeanum, molecular imprinting polymers, preparation, dummy template

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