Determination of Vitamin B12 in Formula foods by High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry

Abstract

Abstract: A method based on high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) was developed for determination of vitamin B12 in formula foods. The samples were purified by HLB solid phase extraction column after pretreatment, then separated on a C18 reversed phase chromatography column by isometric elution using a mobile phase consisting of methanol and 8 mM ammonium acetate aqueous solution ( 19:81，V/V)，Vitamin B12 was determined indirectly with the detection of cobalt ions in samples by ICP-MS. The results showed that the analysis can be completed within 7 min, and the target compound has a good peak type. A good linearity was obtained in the range of 0.1～500 μg/L for vitamin B12, and the correlation coefficients was 0.999. The limits of detection (LOD) was 0.02 μg/kg. The average recoveries of the method was ranged from 81.3 % to 103.6 % with relative standard deviations ranged from 0.4 % to 4.6 % in different food matrix at three spiked levels (n = 6). The method is simple, fast, sensitive, strong resistance to Matrix interference, and wide dynamic range. It can be used for the determination of vitamin B12 in various formula foods.

Key words: high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS), formula food, vitamin B12

CLC Number:

O657.63

References

[1]付炎, 李力更, 王于方, 等. 天然药物化学史话:维生素B12[J]. 中草药, 2015, 46(9):1259-1264. DOI: 10.7501/j.issn.0253-2670.2015.09.001
[2] CASTELLI M C C, WONG D F, FRIEDMAN K, et al. Pharmacokinetics of Oral Cyanocobalamin Formulated With Sodium N-[8-(2-hydroxybenzoyl)amino]caprylate (SNAC): An Open-Label, Randomized, Single-Dose, Parallel-Group Study in Healthy Male Subjects[J]. Clinical Therapeutics, 2011, 33(7): 934-945. DOI: 10.1016/j.clinthera.2011.05.088
[3]马颖清, 王霞, 张小刚, 等. 超高效液相色谱-串联质谱测定营养强化食品中维生素B12 的含量[J]. 上海大学学报（自然科学版）, 2014, 20(1):1-6. DOI: 10.3969/j.issn.1007-2861.2013.07.016
[4] NAKOS M, PEPELANOVA I, BEUTEL S, et al. Isolation and analysis of vitamin B12 from plant samples [J]. Food Chemistry, 2017, 216:301-308. DOI:10.1016/j.foodchem.2016.08.037
[5]王国玲, 邵立君, 聂宏骞, 等. B 族维生素片中维生素B12 电感耦合等离子体质谱法间接测定[J]. 中国公共卫生, 2018, 34(3):462-464. DOI:10.11847/zgggws1114114
[6] EITENMILLER R R, YE L, LANDEN W O. Vitamin analysis for the health and food sciences[J]. Trends in Food Science & Technology, 2009, 20(11-12):598-599. DOI:10.1016/j.tifs.2009.10.005
[7]中华人民共和国卫生部. 食品安全国家标准食品营养强化剂使用标准: GB 14880-2012[S]. 北京: 中国标准出版社, 2012.
[8]中华人民共和国卫生部. 食品安全国家标准婴幼儿食品和乳品中维生素B12的测定:GB 5413.14-2010[S]. 北京: 中国标准出版社, 2010
[9]中华人民共和国卫生部, 中国国家标准化管理委员会. 保健食品中维生素B12的测定: GB/T 5009.217-2008[S]. 北京: 中国标准出版社, 2008
[10]李少旦, 刘韧, 李木兰. 石墨炉原子吸收光谱法间接测定保健食品中维生素B12[J]. 理化检验-化学分册, 2009, 45(4): 422-424.
[11] ZHU J K, SHU L, WU M, et al. investigation on chemiluminescence detection of vitamin B12 with capillary electrophoresis[J]. journal of east china normal university(Natural science) , 2013, 5:96-101. DOI:10.3969/j.issn.1000-5641.2013.05.012
[12] WANG X, LI K F, YAO L P, et al. Recent advances in vitamins analysis by capillary electrophoresis[J]. Journal of Pharmaceutical and Biomedical Analysis, 2018, 147:278-287. DOI:10.1016/j.jpba.2017.07.030
[13]李江, 綦艳, 田秀梅, 等. 酶联免疫法检测婴幼儿配方奶粉中的维生素B12[J]. 食品工业, 2017, 38(8)250-252.
[14] DANIELA H, SETAREH S F, JOSEPH M, et al. Competitive chemiluminescent enzyme immunoassay for vitamin B12 analysis in human milk [J]. Food Chemistry, 2014, 153:60-65. DOI:10.1016/j.foodchem.2013.12.033
[15] JOSé M C, LUIS C, FERNANDO S, et al. Reference values for serum folate and vitamin B12 in a Spanish population using an electrochemiluminiscent method [J]. Revista del Laboratorio Clínico, 2018, 11(3): 118-122. DOI:10.1016/j.labcli.2017.11.002
[16] MOHAMMAD H P, ELMIRA A, JALA A, et al. Highly sensitive and selective electrochemical sensor for detection of vitamin B12 using an Au/PPy/FMNPs@TD-modified electrode[J]. Sensors and Actuators B: Chemical, 2018, 261(15):335-344. DOI:10.1016/j.snb.2018.01.168
[17] GUGGISBERG D, RISSE M C, HADOM R. Determination of Vitamin B12 in meat products by RP-HPLC after enrichment and purification on an immunoaffinity column[J]. Meat Science, 2012, 90(2):279-283. DOI:10.1016/j.meatsci.2011.07.009
[18]徐硕, 金鹏飞, 邝咏梅, 等. HPLC 法测定复合维生素B 片中的微量叶酸、维生素B12和生物素[J]. 药物分析杂志, 2018,38(6):1091-1097. DOI:10.16155/j.0254-1793.2018.06.25
[19]周黎, 余以刚, 徐红, 等. 免疫亲和柱-超高效液相色谱法测定维生素饮料中维生素B12[J]. 中国食物与营养, 2018, 24(3): 27-29.
[20]宁霄, 金绍明, 刘雅丹, 等. 超高效液相串联质谱法同时测定保健食品中10 种水溶性维生素[J]. 中国药事, 2017, 31(4): 392-402. DOI:10.16153/j.1002-7777.2017.04.008
[21] LUCIA D U, YANG L, DING J F, et al. Determination of cyanocobalamin by isotope dilution LC-MS/MS[J]. Analytica Chimica Acta, 2017, 990:103-109. DOI:10.1016/j.aca.2017.07.065
[22] RAGHUPATHI R A, LAKSHMI P R, PRATHYUSHA C, et al. Sensitive LC-MS/MS method for quantification of vitamin B12 in rat serum:Application to pharmacokinetic study [J]. Drug Metabolism and Pharmacokinetics, 2018, 33(1):62-S63. DOI:10.1016/j.dmpk.2017.11.211
[23] OSTERINK J E, NANINCK F G, KOROSI A, et al. Accurate measurement of the essential micronutrients methionine, homocysteine, vitamins B6, B12, B9 and their metabolites in plasma, brain and maternal milk of mice using LC/MS ion trap analysis[J]. Journal of Chromatography B, 2015, 998-999: 106-113. DOI:10.1016/j.jchromb.2015.07.008
[24]刘德晔, 谷静, 马永建, 等. 尺寸排阻色谱电感耦合等离子体质谱联用测定功能饮料、多维片及奶粉中维生素B12[J]. 分析化学, 2014, 42(2):197-202. DOI:10.3724 /SP.J.1096.2014.31079
[25] [Z]WENZEL R, MAJOR D, HESP K, et al. Determination of Vitamin B12 in Equine Urine by Liquid Chromatography-Inductively Coupled-Plasma Mass Spectrometry[J]. Journal of Trace Elements in Medicine and Biology,2018, In Press, Accepted Manuscript DOI:10.1016/j.jtemb.2018.05.005
[26] RAJU C S K, YU L L, SCHIEL J E, et al.A simple and sensitive LC-ICP-MS method for the accurate determination of vitamin B12 in fortified breakfast cereals and multivitamin tablets[J]. Anal. At. Spectrom. 2013, 28: 901-907.
[27] YANES E G, NANCY J, MILLER I.Use of a parallel path nebulizer for capillary-based microseparation techniques coupled with an inductively coupled plasma mass spectrometer for speciation measurements [J]. Spectrochimica Acta Part B, 2004, 59(6): 883-890. DOI:10.1016/j.sab.2004.03.005