Abstract: Objective: To explore the purification and properties of superoxidae dismutase (SOD) from Zophobas morio L. larvae. Methods: SOD were obtained from Zophobas morio L. larvae by salting-out, DEAE-Sepharose FF column chromatography and Sephadex G-75 column chromatography. SDS-PAGE was used to determine its molecular weight and atomic absorption spectrometer was used to identify it types. Results: SOD with highly specific activity was obtained. The purification factor was 68.54. The purified SOD was identified as Cu, Zn-SOD with molecular weight of 37.30 kD. It had an absorption peak at 278 nm and good stability. Its optimal temperature and pH were 40 ℃ and 6.0, respectively. Its high sensitivity to H2O2 and β-mercaptoethanol was found , while a mixture of CHCl3 and CH3CH2OH (3:5, V/V) had little effect on its activity. Conclusion: The SOD extracted from Zophobas morio L．larvae has high enzymatic activity, stability and application potential.

Key words: Zophobas morio L., SOD, purification, characterization