Optimization of Rapeseed Peptide Production by Mixed Solid-State Fermentation

doi:10.7506/spkx1002-6630-201211049

Abstract

Abstract: Based on peptide yield, nitrogen solubility index (NSI) and glucosinolate degradation rate, the production conditions for rapeseed peptide by mixed solid-state fermentation using Bacillus subtilis and Actinomucor elegans preliminarily determined by one-factor-at-a-a-time method and further optimized by response surface analysis coupled with Box-Behnken design. The optimal fermentation conditons were 4:1 of Bacillus subtilis-to-Actinomucor elegans ratio, 32.1 ℃ of fermentation temperature, 4.64 d of fermentation time, resulting in a peptide yield of 9.85% and a glucosinolate degradation rate of 67.83%.

Key words: mixed solid-state fermentation, rapeseed peptide, process conditions, response surface methodology

CLC Number:

TQ936.16

JUXing-rong，WANGXue-feng，HERong，WANGLi-feng，YUANJian. Optimization of Rapeseed Peptide Production by Mixed Solid-State Fermentation[J]. FOOD SCIENCE, 2012, 33(11): 231-236.

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Optimization of Rapeseed Peptide Production by Mixed Solid-State Fermentation

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