Abstract: An analytical method to determine pefloxacin (PEFX) residue in urine and serum was established based on the fact that the presence of Tb3+ enhances the luminous intensity of Ce(Ⅳ)-SO32-- PEFX system. There was a good linear relationship between the luminous intensity of the system and PEFX concentration over the range of 2 × 10-9－6 × 10-7 g/mL. The limit of detection for PEFX was 8.8 × 10-10 g/mL. The relative standard deviation for 11 parallel determination of PEFX at 1 × 10-7 g/mL was 3.1%. The average spike recoveries of PEFX from human urine and duck serum ranged from 98.5% to 102.5%.

Key words: flow injection chemiluminescence, pefloxacin, terbium, urine, serum