Rapid Preparation of Cyanidin-3-glucoside from Mulberry Fruit by Preparative Medium Pressure Liquid Chromatography

doi:10.7506/spkx1002-6630-20180108-094

FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (3): 94-100.doi: 10.7506/spkx1002-6630-20180108-094

• Food Engineering • Previous Articles Next Articles

Rapid Preparation of Cyanidin-3-glucoside from Mulberry Fruit by Preparative Medium Pressure Liquid Chromatography

RAN Guojing1, JIANG Xinwei1, LI Haoyi2, CHEN Junliang1, LI Xusheng1, SUN Jianxia2, BAI Weibin1,*

(1. Guangdong Engineering Technology Center of Food Safety Molecular Rapid Detection, Department of Food Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, China; 2. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510090, China)

Online:2019-02-15 Published:2019-03-05

Abstract

Abstract: Rapid large-scale preparation of monomeric anthocyanins has always been a problem in the industrialization of anthocyanins. Preparative medium pressure liquid chromatography (MPLC) has great potential for application in the food industry. Cyanidin-3-glucoside (C3G) was purified by preparative MPLC using a C18 column from the anthocyanins extracted from mulberry fruit, containing only a few kinds of anthocyanins. The results showed that a total of three chromatographic peaks were separated. The second peak was confirmed to be composed of cyanidin-3-rutinoside (C3R) and C3G by high performance liquid chromatography (HPLC) and mass spectrometry (MS), and C3G accounted for 73.56% of the total amount as calculated by the peak area normalization method. The purity of C3G was over 98% when the peak was collected in several fractions, and a total of 650 mL of C3G was obtained in one operation. The preparative MPLC method has the advantages of large sample loading volume, simple operation and low cost and can provide a useful tool for rapid and large-scale production of C3G.

Key words: mulberry, anthocyanin, preparative medium pressure liquid chromatography, cyanidin-3-glucoside, fractional collection

CLC Number:

TS255.1

RAN Guojing, JIANG Xinwei, LI Haoyi, CHEN Junliang, LI Xusheng, SUN Jianxia, BAI Weibin. Rapid Preparation of Cyanidin-3-glucoside from Mulberry Fruit by Preparative Medium Pressure Liquid Chromatography[J]. FOOD SCIENCE, 2019, 40(3): 94-100.

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Rapid Preparation of Cyanidin-3-glucoside from Mulberry Fruit by Preparative Medium Pressure Liquid Chromatography

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