FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (11): 95-101.doi: 10.7506/spkx1002-6630-20180606-079

• Food Engineering • Previous Articles     Next Articles

Effect of Freeze-Thaw Cycles on Changes in Functional and Structural Properties of Myofibrillar Protein from Mirror Carp (Cyprinus carpio var. specularis)

DENG Siyang, WANG Bo, LI Haijing, ZHONG Qiang, DONG Chunhui, XIA Xiufang*   

  1. School of Food Science, Northeast Agricultural University, Harbin 150030, China
  • Online:2019-06-15 Published:2019-06-28

Abstract: The effect of freeze-thaw cycles on the functional and structural properties of myofibrillar protein from mirror carp (Cyprinus carpio var. specularis) was investigated by measuring the changes in the emulsifying properties, solubility, δ-potential, surface hydrophobicity and content of sulfydryl, free amino acids, dityrosine, surface hydrophobicity and α-helix. The results indicated that the solubility, emulsifying activity and emulsion stability of myofibrillar protein decreased to 62.85%, 20.67 m2/g and 34.83%, respectively after five freeze-thaw cycles (P < 0.05). The δ-potential, surface hydrophobicity and dityrosine content increased constantly with the increase of freeze-thaw cycles, while the sulfydryl, free amino acid and α-helical contents decreased gradually. These changes suggested that freeze-thaw cycles damaged the structural integrity and functional properties of fish myofibrillar protein.

Key words: freeze-thaw cycle, Cyprinus carpio var. specularis, myofibrillar protein, functional properties, structure

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