Abstract: In this research, we developed an on-line solid phase extraction (SPE) combined with liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the detection of arbidol, amantadine, rimantadine, memantine and oseltamivir in chicken meat and eggs. Samples were extracted with 1.0% formic acid in acetonitrile, and the extract was purified using an MCX on-line solid phase extraction cartridge. The target substances were separated using an XBridge C18 column, detected by electrospray ionization in the positive ion mode, and quantitated by the internal standard method. The results showed that the calibration curves of all analytes had a good linear relationship in the concentration range of 0.05–100 ng/mL. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.05–0.10 μg/kg and 0.10–0.20 μg/kg, respectively. The recoveries of the developed method were in the range of 80.03%–93.96%, with relative standard deviations (RSDs) of lower than 10%. The method was proven to be simple and sensitive, and could be used for the qualification and quantification of the five antiviral drugs in chicken meat and eggs.

Key words: on-line solid phase extraction; liquid chromatography-tandem mass spectrometry; antiviral drugs; internal standard quantification