FOOD SCIENCE ›› 2022, Vol. 43 ›› Issue (12): 194-198.doi: 10.7506/spkx1002-6630-20210818-232

• Bioengineering • Previous Articles    

Isolation and Identification of Lactobacillus sakei from Fermented Mandarin Fish and Its Application in Fermented Mandarin Fish

ZHOU Yingqin, SUN Ziyi, HUANG Jingjing, YAN Yan, ZHENG Haibo, XIE Ningning   

  1. (1. Institute of Agro-product Science and Technology, Anhui Academy of Agricultural Sciences, Hefei 230031, China; 2. Anhui Engineering Laboratory for Functional Microorganisms and Fermented Foods, Hefei 230031, China; 3. College of Food Engineering, Anhui University of Science and Technology, Chuzhou 239000, China)
  • Published:2022-07-01

Abstract: A Lactobacillus strain named SMF-L5 from traditional fermented mandarin fish was isolated using MRS medium with bromocresol green and identified as Lactobacillus sakei by morphological, physiological and biochemical assays and 16S rDNA sequence analysis. L. sakei SMF-L5 grew well in MRS liquid medium, entered the stable growth period after 14 h, and produced the highest viable cell count of 7.0 × 109 CFU/mL. The production of lactic acid of the strain was 10 g/L after 24 h cultivation. The optimal culture conditions were as follows: culture temperature 30.9 ℃, initial pH 6.15 and inoculum amount 1.94%. Strain SMF-L5 had tolerance to 0.08 g/mL NaCl, and obvious inhibitory effects on Escherichia coli, Staphylococcus aureus. The fermented mandarin fish inoculated with L. sakei SMF-L5 had superior color, texture and flavor than spontaneously fermented mandarin fish. These results showed that L. sakei SMF-L5 has the potential for application as an excellent starter in the industrial production of smelly mandarin fish .

Key words: fermented mandarin fish; Lactobacillus sakei; isolation and identification; biological characteristics; inoculated fermentation

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