Abstract: Two short peptides PF1 and PF2 with isoelectric points of 12.01 and 3.18, respectively, were designed and synthesized artificially, with full gene length of 309 bp. Each gene was amplified with two primers, cloned into the pET-30a (+) vector and expressed. The expressed products were purified by Ni-NTA column affinity chromatography. SDS-PAGE and Western blot analysis showed that the expressed target protein was consistent with the expected size. The purified short peptides PF1 and PF2 increased and decreased glucose oxidase (GOD) activity by 8.34% and 6.88%, respectively. Therefore, it could be concluded that artificially designed short peptides with different charges PF1 and PF2 can promote or inhibit the catalytic effect of GOD, and further broaden the application scope of GOD in food fermentation and food preservation.

Key words: glucose oxidase; charged short peptide; cloning and expression