Preparation and Immunological Characterization of Monoclonal Antibody against Ochratoxin A

doi:10.7506/spkx1002-6630-201109053

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (9): 236-240.doi: 10.7506/spkx1002-6630-201109053

• Bioengineering • Previous Articles Next Articles

Preparation and Immunological Characterization of Monoclonal Antibody against Ochratoxin A

SUN Ya-ning1,2，TENG Man1，HU Xiao-fei1，HOU Yu-ze2，CHAI Shu-jun1，LIU Qing-tang1，ZHI Ai-min1，WANG Lei2，ZHANG Gai-ping1，DENG Rui-guang1,*

1. Key Laboratory of Animal Immunology of Ministry of Agriculture, Henan Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China；2. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China

Online:2011-05-15 Published:2011-04-11

Abstract

Abstract: In this paper, immunogen OTA-BSA was prepared by EDC method and used to immunize mice for constructing a hybridoma cell line with the ability to secrete anti-OTA monoclonal antibody (anti-OTA mAb). The preparation of ascites was carried out using in vivo induction method. Immunological properties such as subclass, titer, sensitivity, affinity and specificity of the purified mAb were characterized. The mAb against OTA was type IgG1, with ELISA titer of 1: 6.4 × 105, IC50 value of 112.8 pg/mL, affinity constant (Ka) of 9.74×1011 L/mol, cross-reactivity to OTB and other fungus metabolites of 5.6% and less than 0.003%, respectively. The anti-OTA mAb was characterized with high titer, sensitivity and specificity and can thereby be used for rapid immunological detection of OTA.

Key words: ochratoxin A(OTA), monoclonal antibody, sensitivity, specificity

CLC Number:

Q785

SUN Ya-ning，TENG Man，HU Xiao-fei，HOU Yu-ze，CHAI Shu-jun，LIU Qing-tang，ZHI Ai-min，WANG Lei，ZHANG Gai-ping，DENG Rui-guang. Preparation and Immunological Characterization of Monoclonal Antibody against Ochratoxin A[J]. FOOD SCIENCE, 2011, 32(9): 236-240.

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Preparation and Immunological Characterization of Monoclonal Antibody against Ochratoxin A

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