Principle and Application of Trans-Cleavage CRISPR/Cas Technology for the Detection of Foodborne Pathogenic Microorganisms

doi:10.7506/spkx1002-6630-20231205-029

FOOD SCIENCE ›› 2024, Vol. 45 ›› Issue (22): 351-360.doi: 10.7506/spkx1002-6630-20231205-029

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Principle and Application of Trans-Cleavage CRISPR/Cas Technology for the Detection of Foodborne Pathogenic Microorganisms

DAI Yongjin, SUN Jing, ZHANG Moran, LIU Yujuan, CHEN Hongzhou, LU Yingjian

(1. College of Food Science and Engineering, Nanjing University of Finance and Economics, Modern Grain Circulation and Security Collaborative Innovation Center, Nanjing 210023, China; 2. Anhui Guotai Zhongxin Testing Technology Co. Ltd., Hefei 230051, China)

Online:2024-11-25 Published:2024-11-05

Abstract

Abstract: Foodborne pathogenic microorganisms are a serious threat to food safety. Currently, traditional biochemical assays are facing a series of challenges in the detection of foodborne pathogens. In recent years, with the gradual development of CRISPR (clustered regularly interspaced short palindromic repeats) technology, the CRISPR/CRISPR-associated proteins (Cas) system with trans-cleavage activity has received much attention from researchers. In this article, the origin, classification and principles of CRISPR/Cas systems with trans-cleavage activity are summarized. This paper also reviews the versatile application of trans-cleavage CRISPR/Cas systems in the field of foodborne pathogens, and concludes with a discussion of the advantages, disadvantages and future directions of this technology.

Key words: clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins systems; trans-cleavage; foodborne pathogen; nucleic acid detection

CLC Number:

TS201.6

DAI Yongjin, SUN Jing, ZHANG Moran, LIU Yujuan, CHEN Hongzhou, LU Yingjian. Principle and Application of Trans-Cleavage CRISPR/Cas Technology for the Detection of Foodborne Pathogenic Microorganisms[J]. FOOD SCIENCE, 2024, 45(22): 351-360.

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Principle and Application of Trans-Cleavage CRISPR/Cas Technology for the Detection of Foodborne Pathogenic Microorganisms

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