FOOD SCIENCE ›› 2025, Vol. 46 ›› Issue (16): 175-170.doi: 10.7506/spkx1002-6630-20241227-237
• Bioengineering • Previous Articles
HUAN Jiaxin, HUANG Yanping, NIU Yanzhe, SONG Hong, LIU Manshun, YUAN Chunlong
Published:
Abstract: To enhance the stability of Amano lipase A (ANL) and its degradation efficiency toward ochratoxin A (OTA), ANL was immobilized on a Zn-metal-organic framework (Zn-MOF) carrier by a one-step in situ method, yielding ANL@Zn-MOF. Based on OTA degradation rate, the optimal immobilization conditions were determined as 8 mg, 2 h and 400 r/min for enzyme loading, immobilization time, and stirring speed, respectively. ANL@Zn-MOF was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). The results confirmed the successful loading of ANL onto Zn-MOF. ANL@Zn-MOF exhibited superior alkali tolerance, thermal stability, and ionic strength stability compared with free ANL. ANL@Zn-MOF degraded OTA more rapidly. Specifically, it (containing 20 ng/mL ANL) completely degraded OTA in 8 h, which was shortened by 66.7% compared with free ANL. Moreover, in a 500 mL reaction system, ANL@Zn-MOF degraded 69.3% of OTA in 12 h. Additionally, ANL@Zn-MOF exhibited excellent reusability, retaining 78% of its activity after five cycles of reuse. This study establishes a foundation for the application of ANL@Zn-MOF in degrading OTA in food systems and provides a reference for the use of MOFs in enzyme immobilization.
Key words: Amano lipase A; metal-organic framework; immobilization; ochratoxin A degradation
CLC Number:
Q814.2
HUAN Jiaxin, HUANG Yanping, NIU Yanzhe, SONG Hong, LIU Manshun, YUAN Chunlong. Efficient Degradation of Ochratoxin A by Zn-Metal-Organic Framework Immobilized Lipase[J]. FOOD SCIENCE, 2025, 46(16): 175-170.
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URL: https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-20241227-237
https://www.spkx.net.cn/EN/Y2025/V46/I16/175