Abstract: Carbamate pesticides are widely used as insecticides, acaricides, and fungicides due to their broad bioactivity, low cost, and high efficacy. However, they are potential carcinogens and mutagens, with some posing severe neurotoxic risks to the central nervous system, leading to serious health issues such as headaches and memory loss. In this study, based on the structural features of seven carbamate pesticides, a urea-functionalized magnetic covalent organic framework (COF) material was synthesized using 4′,5′-bis(4-aminophenyl)-[1,1′:2′,1″-terphenyl]-4,4″-diamine (BATD) and 1,4-diisocyanatobenzene (PPDI) as monomers. The COF material was used as a sorbent for magnetic solid-phase extraction (MSPE) of carbamate pesticide residues.Characterization methods, including scanning electron microscopy (SEM) and transmission electron microscopy (TEM), confirmed the successful modification of the material onto Fe?O? nanoparticles. The extraction conditions were optimized, and the MSPE material was coupled with ultra-high-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for the enrichment and detection of carbamate pesticide residues in Lonicera japonica (honeysuckle) and Chrysanthemum morifolium (chrysanthemum). The analysis method demonstrated good linearity (R2 > 0.9962) in the range of 2–100 ?g/kg, with recovery rates between 76.90% and 109.86%. The limits of detection were 0.03–0.6 ?g/kg, and the limits of quantification were 0.1–2.0 ?g/kg, confirming the method's sensitivity and accuracy for the detection of carbamate residues in these herbal samples.?

Key words: covalent organic framework materials(COFs), ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS), magnetic solid-phase extraction(MSPE), carbamate pesticides