A Comparative Study of Post-column Derivatization HPLC and LC-MS/MS Determination of Carbamate Pesticide Residues in Vegetables

doi:10.7506/spkx1002-6300-201002047

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (2): 183-186.doi: 10.7506/spkx1002-6300-201002047

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A Comparative Study of Post-column Derivatization HPLC and LC-MS/MS Determination of Carbamate Pesticide Residues in Vegetables

HAO Xue-fei，DONG Xiao-hai，ZHONG Hong-jian

Research Center of Agricultural Quality Standards and Testing Techniques, Henan Academy of Agricultural
Sciences, Zhengzhou 450002

Received:2009-03-25 Revised:2009-08-19 Online:2010-01-15 Published:2010-12-29
Contact: HAO Xue-fei， E-mail:haoxuefei926@yahoo.com.cn

Abstract

Abstract:

In the present study, 7 carbamate pesticides in vegetables were determined using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), and this method was compared with commonly used HPLC with post-column derivatization method. Results showed that the analytes were well separated using both methods, which had good figures of merit. HPLC with post-column derivatization method is considered as a classical method having the advantage of good repeatability and the disadvantages of time-consuming operation, expensive derivative reagent cost and poor stability; in contrast, LCMS/MS, thanks to its short retention time, high sensitivity, simple and easy operation, and rapidity, is more competitive for determination of carbamate pesticides in vegetables.

Key words: carbamate pesticides residues, HPLC, LC-MS/MS, comparison

CLC Number:

TQ450.263

HAO Xue-fei，DONG Xiao-hai，ZHONG Hong-jian. A Comparative Study of Post-column Derivatization HPLC and LC-MS/MS Determination of Carbamate Pesticide Residues in Vegetables[J]. FOOD SCIENCE, 2010, 31(2): 183-186.

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A Comparative Study of Post-column Derivatization HPLC and LC-MS/MS Determination of Carbamate Pesticide Residues in Vegetables

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